Inflammatory cytokine IL-10 polymorphism in patients of ulcerative colitis and irritable bowel syndrome

Abstract

S 21 ANNUAL CONFERENCE—2013 M isella n eo u s Corresponding author: Jitendra Km Choudhary. E-mail: jitendra709@gmail.com INFLAMMATORY CYTOKINE IL-10 POLYMORPHISM IN PATIENTS OF ULCERATIVE COLITIS AND IRRITABLE BOWEL SYNDROME Manish Kumar Tripathi, Ashok Kumar Jain, Vinod Kumar Dixit, Pankaj Kaushik, Jitendra Kumar Choudhary, Manjita Srivastava, Neha Singh, Arttrika Ranjan, Neha Gupta, Sangey Chopel Lamtha, Smita Verma Department of Gastroenterology, Institute of Medical Sciences, BHU, Varanasi Background: Ulcerative colitis (U.C.) is a complex multifactorial trait involving both environmental and genetic factors. UC is characterized by activation of macrophages and T lymphocyte; pro-inflammatory cytokine, chemokines and adhesion molecule expression and an inability to adequately down-regulate immune activation. Aim: The aim of the present study is to examine Interleukin 10 gene polymorphism in patients with UC and IBS. Methods: Age and sex matched 56 patients of IBS and 36 patients of ulcerative colitis were enrolled in the study. Blood samples were obtained after informed consent for DNA isolation by phenol-chloroform method. PCR for IL 10 -1082, -819, -592 was done followed by restriction digestion by site specific enzyme Mnl1, RsaI and MaeIII respectively, the results was analyzed on 2% agarose gel. Results: The percentage distribution of genotypic frequencies of IL-10 -1082 homozygotes and heterozygotes are given in the table 1. Table 1 Genotypic frequencies of IL10 -1082, -819, -592 between IBS and UC patients Cytokines Genotype Frequency IL-10 (1082) AA AG GG IBS 32(57.1) 16(28.6) 8(14.3) Ulcerative Colitis 22(61.1) 8(22.2) 6(16.7) c2=0.479, p=0.787 IL-10 (819) TT TC CC IBS 13(23.2) 21(37.5) 22(39.3) Ulcerative Colitis 4(11.1) 20(55.6) 12(33.3) c2=3.550, p=0.169 IL-10 (592) AA AC CC IBS 36(64.3) 10(17.9) 10(17.9) Ulcerative Colitis 16(44.4) 12(33.3) 8(22.2) c2=3.934, p=0.140 S124 Conclusion: Genotypic frequencies of IL-10 between UC and IBS patients showed that there is no statistically significant difference in IL-10 -1082, -819, -592 cytokine genes. Genotype ATA/ATA correlate with low level of IL-10 production Hence the results of this study indicate low production of anti-inflammatory cytokines IL-10 in patients with UC and IBS. Corresponding author: V.K.Dixit. E-mail: manish.imsbhu@gmail.com