Abstract
Background: Toll-like receptor-2 (TLR2) is highly important within the immune system. Characterization of the expression of TLR2 within inflammatory cells in periapical lesions could help in diagnosis and management of refractory cases. The aim of the study is identification of Toll-like receptor (TLR2) through immunohistochemical and immunofluroscence expression in inflammatory cells within refractory periapical granuloma cases. Methods: Eight cases of refractory periapical granuloma were selected out of 772 cases. Histological examination and immunohistochemical staining with polyclonal rabbit antihuman TLR2, monoclonal mouse antihuman CD38, CD68 and CD83 primary antibodies, as well as immunofluorescence staining with goat anti-rabbit TLR2, donkey anti-mouse CD38, CD68 and CD83 primary antibodies was conducted. Positive controls, negative controls and experimental sections with no primary antibody were included in the study. Qualitative analysis and double immunofluorescence technique was used to characterize the TLR + cells. Results: In periapical granuloma, lymphocytes (CD38 cells) expressed the most amount of TLR reactivity followed by macrophages (CD68 cells), and odontogenic epithelial cells. Neutrophils, red blood cells (RBCs) and collagen ground substance were negative to TLR2. Conclusion:TLR2 was highly expressed by lymphocytes and plasma cells indicative of their major role in the inflammatory process and antigen recognition in refractory periapical granuloma. Dendritic cells expressing TLR2 were low in number suggesting a minor role in sustaining these lesions.
Highlights
The goal of conventional root canal treatment is to eliminate root canal microbes, or to substantially reduce microbial load and prevent re-infection by root canal filling and coronal seal
All tissue sections showed characteristics of granulation tissue (Figure 1a, b, c), typically mature fibrous connective tissue with a moderately intense infiltrate of chronic inflammatory cells dominated by lymphocytes
Our results showed that neutrophils, red blood cells (RBCs), the collagen ground substance and the negative control samples from periapical scar tissues showed a negative reaction to immunostaining with Toll-like receptor-2 (TLR2) antibody
Summary
The goal of conventional root canal treatment is to eliminate root canal microbes, or to substantially reduce microbial load and prevent re-infection by root canal filling and coronal seal When this is achieved, healing of the peri-radicular tissue is expected. Pathogen recognition through innate immunity is achieved by several germline encoded pattern recognition receptors (PRRs). Characterization of the expression of TLR2 within inflammatory cells in periapical lesions could help in diagnosis and management of refractory cases. The aim of the study is identification of Toll-like receptor (TLR2) through immunohistochemical and immunofluroscence expression in inflammatory cells within refractory periapical granuloma cases. Conclusion: TLR2 was highly expressed by lymphocytes and plasma cells indicative of their major role in the inflammatory process and antigen recognition in refractory periapical granuloma. Dendritic cells expressing TLR2 were low in number suggesting a minor role in sustaining these lesions
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
