Abstract
MicroRNA (miRNA) in tissue and liquid samples have been shown to be associated with many diseases including inflammation. We aimed to identify inflammation-related miRNA expression level in the bovine mastitis milk. Expression level of inflammation-related miRNA in milk from mastitis-affected and normal cows was analyzed using qPCR. We found that expression level of miR-21, miR-146a, miR-155, miR-222, and miR-383 was significantly upregulated in California mastitis test positive (CMT+) milk. We further analyzed these miRNA using a chip-based QuantStudio Digital PCR System. The digital PCR results correlated with those of qPCR, demonstrating upregulation of miR-21, miR-146a, miR-155, miR-222, and miR-383 in CMT+ milk. In conclusion, we identified miRNA that are upregulated in CMT+ milk. These miRNA exhibited sensitivity and specificity greater than 80% for differentiating between CMT+ milk and normal milk. Our findings suggest that inflammation-related miRNA expression level in the bovine milk was affected by mastitis, and miRNA in milk have potential for use as biomarkers of bovine mastitis.
Highlights
MicroRNA are small noncoding RNA molecules of 18–25 nucleotides. miRNA regulate gene expression at the post-transcriptional level by causing RNA degradation or blocking mRNA translation. miRNA in tissue and liquid samples have been shown to be associated with many diseases including inflammation [1,2,3]
We identified that miR-21, miR-146a, miR-155, miR-222, and miR-383 were significantly upregulated in California Mastitis Test (CMT)+ milk
Our findings suggest that inflammation-related miRNA expression level in the bovine milk was affected by mastitis, and miRNA in milk have potential for use as biomarkers of bovine mastitis
Summary
MicroRNA (miRNA) are small noncoding RNA molecules of 18–25 nucleotides. miRNA regulate gene expression at the post-transcriptional level by causing RNA degradation or blocking mRNA translation. miRNA in tissue and liquid samples have been shown to be associated with many diseases including inflammation [1,2,3]. MicroRNA (miRNA) are small noncoding RNA molecules of 18–25 nucleotides. MiRNA regulate gene expression at the post-transcriptional level by causing RNA degradation or blocking mRNA translation. MiRNA in tissue and liquid samples have been shown to be associated with many diseases including inflammation [1,2,3]. MiRNA have specific roles in disease and inflammation pathogenesis. MiRNA have been investigated as noninvasive biomarkers of various diseases in humans, including cancer, cardiac disease, infection, and inflammatory disease [4, 5]. MiRNA have been identified in the milk of cows [6, 7], pigs [8], humans [9], goats [10], rats [11], and yaks [12].
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