Abstract
Objective To investigate the expression of Tie 2 gene induced by inflammation cytokines TNF-α and IL-1β in vascular endothelium cells and explore the mechanism of angiogenesis under inflammation. Methods mRNA and protein expressions of Tie 2 were investigated by quantitative PCR and Western blot in primary human aortic endothelial cells (HAECS) and/or primary human pulmonary artery endothelial cells (HPAECS)induced by inflammatory cytokines. Results TNF-α and IL-1β could increased the expression of Tie 2 in primary endothelial cells. The expression of Tie 2 was significantly increased 24 hours after stimulation of TNF-α in HAECS and HPAECS and IL-1β in HPAECS. in HPAECS, the effect of both arrived the peak at 36 hours after stimulation. Conclusion The expression of Tie 2 could be increased by inflammatory cytokines TNF-α and IL-1β in human vascular endothelial cells. It possibly contributes for angiogenesis under inflammation. Key words: TNF-α; IL-1β; Tie 2; endothelium cell
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
