Abstract
BackgroundReduced erythrocyte survival and deformability may contribute to the so-called anemia of inflammation observed in septic patients. Erythrocyte structure and function are affected by both the membrane lipid composition and the organization. We therefore aimed to determine whether these parameters are affected during systemic inflammation. MethodsA sensitive matrix-assisted laser desorption and ionization time-of-flight mass spectrometric method was used to investigate the effect of plasma components of 10 patients with septic shock and of 10 healthy volunteers subjected to experimental endotoxemia on erythrocyte membrane lipid composition. ResultsIncubation of erythrocytes from healthy control donors with plasma from patients with septic shock resulted in membrane phosphatidylcholine hydrolysis into lysophosphatidylcholine (LPC). Plasma from volunteers undergoing experimental human endotoxemia did not induce LPC formation. The secretory phospholipase A2 IIA concentration was enhanced up to 200-fold in plasma of septic patients and plasma from endotoxin-treated subjects, but did not correlate with the ability of these plasmas to generate LPC. Erythrocyte phosphatidylserine exposure increased up to two-fold during experimental endotoxemia. ConclusionsErythrocyte membrane lipid remodeling as reflected by LPC formation and/or PS exposure occurs during systemic inflammation in a secretory phospholipase A2 IIA-independent manner. General significanceSepsis-associated inflammation induces a lipid remodeling of the erythrocyte membrane that is likely to affect erythrocyte function and survival, and that is not fully mimicked by experimental endotoxemia.
Highlights
In patients with inflammation, anemia is associated with poor patient outcome
Using sensitive matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), we investigated the lipid composition of erythrocytes after incubation with the plasma of patients suffering from septic shock and with plasma of subjects undergoing experimental endotoxemia
LPC formation was significantly enhanced in erythrocytes treated with plasma from septic patients, but not in erythrocytes treated with plasma from control donors (Fig. 1D)
Summary
To changes in iron homeostasis and defective erythropoiesis [1,2], reduced erythrocyte lifespan contributes to “anemia of inflammation” [3,4,5] This condition is common in patients suffering from sepsis [6]. Reduced erythrocyte survival and deformability may contribute to the so-called anemia of inflammation observed in septic patients. Conclusions: Erythrocyte membrane lipid remodeling as reflected by LPC formation and/or PS exposure occurs during systemic inflammation in a secretory phospholipase A2 IIA-independent manner. General significance: Sepsis-associated inflammation induces a lipid remodeling of the erythrocyte membrane that is likely to affect erythrocyte function and survival, and that is not fully mimicked by experimental endotoxemia
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