Infiltration of Nicotiana benthamiana Protocol for Transient Expression via Agrobacterium

Abstract

Transient expression in tobacco plant (Nicotiana benthamiana) is used to determine the subcellular location of a protein of interest when tagged with a reporter such as green fluorescent protein (GFP), or to mass produce proteins without making transgenic plants. The root tumor bacteria, Agrobacteria, are used as medium to introduce the target gene expression cassette into benthamiana mesophyll cells., 当用例如绿色荧光蛋白（GFP）的报告物标记时，烟草植物（本塞姆氏烟草）中的瞬时表达用于确定目标蛋白质的亚细胞定位，或大规模生产蛋白质而不进行转基因 植物。 根瘤细菌，土壤杆菌用作培养基以将靶基因表达盒导入本氏体叶肉细胞中。