Inference of genetic networks using random forests: Assigning different weights for gene expression data.

Abstract

In using gene expression levels for genetic network inference, we believe that two measurements that are similar to each other are less informative than two measurements that differ from each other. Given, for example, that gene expression levels measured at two adjacent time points in a time-series experiment are often similar to each other, we assume that each measurement in the time-series experiment will be less informative than each measurement in a steady-state experiment. Based on this idea, we propose a new inference method that relies heavily on informative gene expression data. Through numerical experiments, we prove that the quality of an inferred genetic network is slightly improved by heavily weighting informative gene expression data. In this study, we develop a new method by modifying the existing random-forest-based inference method to take advantage of its ability to analyze both time-series and static gene expression data. The idea we propose can be similarly applied to many of the other existing inference methods, as well.