Abstract

No occurrence of milky disease resulted when third-instar larvae of the Japanese beetle, Popillia japonica, were exposed to soil containing 2 billion spores/kg of strain NRRLB-2309M of the milky disease bacterium, Bacillus popilliae, produced on a laboratory medium, or when the larvae imbibed 200,000 of the spores of strain NRRL B-2309M in aqueous suspension; however, 24% of the larvae were infected when they were injected with 10,000 of the spores. In contrast, spores produced commercially 2

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