Infective capacity of Cryptococcus neoformans and Cryptococcus gattii in a human astrocytoma cell line.

Abstract

Pathogenesis of cryptococcosis in the central nervous system (CNS) is a topic of ongoing research, including the mechanisms by which this fungus invades and infects the brain. Astrocytes, the most common CNS cells, play a fundamental role in the local immune response. Astrocytes might participate in cryptococcosis either as a host or by responding to fungal antigens. To determine the infectivity of Cryptococcusneoformans var. grubii and Cryptococcusgattii in a human astrocytoma cell line and the induction of major histocompatibility complex (MHC) molecules. A glioblastoma cell line was infected with C.neoformans var. grubii and C.gattii blastoconidia labelled with FUN-1 fluorescent stain. The percentage of infection and expression of HLA class I and II molecules were determined by flow cytometry. The interactions between the fungi and cells were observed by fluorescence microscopy. There was no difference between C.neoformans var. grubii and C.gattii in the percentage infection, but C.neoformans var. grubii induced higher expression of HLA class II than C.gattii. More blastoconidia were recovered from C.neoformans-infected cells than from C. gattii infected cells. Cryptococcus neoformans var. grubii may have different virulence mechanisms that allow its survival in human glia-derived cells.