Abstract
SummaryH2O, 1.0 M Na2SO4, and 1.5 M MgSO4 protected the JBE virus from inactivation at 50°C, whereas 0.1 M and 1.0 M NaCI, 0.1 M Na2SO4 as well as 0.15 M MgSO4 inactivated the virus. However, the JBE-RNA was more stable in the presence of a high concentration of NaCl than in H2O and other salts tested. In addition, when EDTA was used either as a solution for homogenization of infected mouse brains or as a suspending solution of extracted infectious JBE-RNA, especially in the former case, the PFU of the RNA which were measured on chick embryo cell monolayers closely approached that of the virus and the infectivity of the RNA was protected from inactivation.
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