Abstract
Introduction The genus Ralstonia was introduced in 1995 to accommodate the generically misplaced species Burkholderia pickettii, Burkholderia solanacearum, and Alcaligenes eutrophus. Ralstonia spp. are isolated from a wide variety of ecologic sites, including plants and soils contaminated with heavy metals (1). Since its introduction, the taxonomy of the genus has expanded to include several species: Ralstonia pickettii (type strain ATCC 27511), R. basilensis, R. campinensis, R. eutropha, R. gilardii, R. insidiosa, R. metallidurans, R. mannitolilytica corrig., R. oxalatica, R. paucula, R. respiraculi, R. syzygii comb. nov., R. solanacearum and R. taiwanensis (2-13). Coeyne et al. described Ralstonia insidiosa sp. nov. The type strain, LMG 21421 (= CCUG 46789), was isolated from sputum of a patient with acute lymphoblastic leukemia in the United States in 2001 (5). R. insidiosa can be differentiated from other species of the genus Ralstonia and phenotypically similar species (including Burkholderia cepacia and Achromobacter xylosoxidans) by a variety of biochemical tests, 16S rDNA gene sequence analysis, whole-cell protein analysis, and several PCRbased assays. This report presents two cases of infection caused by R. insidiosa.
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