Infection status of cervical human papilloma virus in patients with cervical intraepithelial neoplasia

Abstract

To explore the human papilloma virus (HPV) infection and genotypes distribution in female patients with cervical intraepithelial neoplasia so as to provide rationales for preventing and treating HPV and developing HPV vaccine. Polymerase chain reaction (PCR) amplification, flow-through hybridization and gene chip were used to identify 23 HPV genotypes in cervical cells collected from 823 female patients with cervical intraepithelial neoplasia at Third Municipal People's Hospital and Central Municipal Hospital during January 2011 and December 2013. Among them, HPV infections were detected in 47.51% (391/823). From 2011 to 2013, there was a trend of increase year by year and the difference was statistically significant (P < 0.05). With the greater degree of cervical intraepithelial neoplasia, LR-HPV and HR-HPV infection rate increased. And there were statistically significant differences (P < 0.05). And single or double genotype infection dominated (86.19%). Multiple genotype infection accounted for 0.51%. And 76.21% of HPV infections were high-risk genotypes and most of them belonged to HPV16. Otherwise, most low-risk genotypes were HPV6. Besides, HPV39 and HPV82 were not detected. HPV infections, particularly high-risk ones, are widespread among female patients with condyloma acuminatum or cervical erosion in Qingdao. Thus active clinical therapy should be provided.