Abstract

AbstractAlstroemeria cv. Ovation plants with virus‐like necrotic spots and streaks on leaves and petals were observed in greenhouses in Khorasan Razavi (Mashhad) and Markazi (Mahallat) provinces, Iran. Samples with virus‐like symptoms reacted positively in enzyme‐linked immunosorbent assay with a polyclonal antibody raised against Tomato yellow ring virus (TYRV) nucleocapsid (N) protein. TYRV‐specific primers were used in a reverse transcription‐polymerase chain reaction to amplify the N gene. The deduced amino acid sequences of the obtained amplicon revealed 99% identity to the N protein of an isolate of TYRV isolated from tomato (TYRV‐t).

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