Infection density, diversity, and distribution of Wolbachia bacteria in moths (Order Lepidoptera): First systematic report from Thailand

Abstract

• First systematic report on Wolbachia infections in moths from Thailand. • Surveys in various habitats from 5 provinces in 3 geographic regions of Thailand. • Wolbachia were detected in 28 of 58 moth species and 625 of 1235 individuals. • Some moth species had not been described previously as infected. • Most specimens were collected from the West region of Thailand. Members of the genus Wolbachia are a group of Rickettsia -like, intracellular, maternally inherited bacterial endosymbionts that infect a diverse range of insects and cause reproductive changes in their hosts. Although Wolbachia ’s role in many insects has been extensively studied, only a little of their effects on host’s reproduction and their infection frequencies were reported in Lepidopteran which is one of the most diverse insects. Here, we present the first systematic survey of the Wolbachia infection status in different species of moths from three different geographic regions of Thailand, which was carried out during January to December in 2019 with the screening of 1,235 specimens in total of 58 moth species from 13 families. Specimens were collected from Khao Yai National Park (the Central and the Northeast regions), and Kaeng Krachan National Park (the West region). Infections of Wolbachia were screened by using polymerase chain reaction with 16S rRNA, ftsZ and wsp gene primers which the results indicated high rates of Wolbachia infection in moth populations from Thailand. Wolbachia was found in all different geographically populations in total of 625 individuals in total of 28 moth species from 9 families, including 144 individuals (46 males and 98 females) from the Central, 156 individuals (49 males and 107 females) from the Northeast, and 325 individuals (114 males and 211 females) from the West. The highest infection rate was 90.47% in the West populations and the average infection rate was 61.90%. The detection of Wolbachia in different moth populations from all regions was identical when all primers were used to screen for Wolbachia . The relative densities of Wolbachia within each individual were determined using quantitative real-time PCR and the result showed that there was a low Wolbachia infection density in these moth populations. These findings indicated that Wolbachia are distributed throughout the moth populations from Thailand.