Abstract

Nosocomial Infections are the infections that are neither at incubating state nor present at the time of admission. They are greatly associated with Surgical procedures and Invasive Medical Devices and hence OTs and ICUs are known as ‘Hot Zones’ and Gram Negative and Gram-positive organisms are responsible. Hence, regular surveillance of these areas remains the mainstay to monitor the raising trend of nosocomial infections. To find out the prevalence of Bacteria and Bacterial load in Pre and Post fumigation swabs from various areas of Maternity and Children hospital like OTs, Patient Ward, Labor ward and NICUs and to test the effectiveness of the standard disinfectants. The swabs were collected from different areas of MGM Maternity and Children Hospital, Kalamboli Mumbai The swabs were further processed and organisms were identified using standard Microbiological procedures. The standard disinfectants were tested using Kelsey-Sykes Capacity test, and they “Passed” the test. Out of the total Pre fumigation swabs collected, growth was seen in 15% of the swabs while in Post fumigation swabs 3% of growth was seen. In places like Minor OT, Septic OT, Labor Room, Waiting Room a higher value of Chi-square test statistics indicated a statistically significant association between fumigation and growth of microorganisms. Majorly non-pathogenic organism like Bacillus was isolated followed by CoNS and few . Hence this indicates significant reduction in growth of microorganisms in majority of the areas of post fumigation swabs indicating following of proper infection control procedures. This study describes the profile of Bacterial Load in high-risk areas of Maternity and Children Hospital. Pre and post fumigation swabs were tested from high-risk areas including Minor OT, Major OT, Septic OT, Female Ward, Recovery Room, PNC, NICU. Also, suggestions were made to incorporate air sampling methods for better assessments. The knowledge of these findings and appropriate corrective measures can greatly impact the health of neonates as well as the mothers.

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