Abstract
Infection and multiplication of Leishmania infantum and L. mexicana inside of skin fibroblasts from hamsters, mice and rats was achieved. This process was demonstrated either by counting parasites inside the stained cells or by electronic microscopy studies. In addition multiplication rate differences in the cells from these rodent species were determined, for L. infantum as well as for L. mexicana. Parasite development in hamsters and mice fibroblasts was evident but there was not multiplication in rat cells showing that apparently they are refractory to Leishmania infection. These results suggest that the parasite affinity for each animal, as well as any intracellular environment resistance, could involve genetic factors in the parasite multiplication. On the other hand, presence of amastigote multiplication inside of parasitophorus vacuole, showed by electronic microscopy images, probes a true parasite transformation. Therefore it is suggested that fibroblasts could work as host cells for parasite survival and permanency in the infected animals.
Highlights
Infection and multiplication of Leishmania infantum and L. mexicana inside of skin fibroblasts from hamsters, mice and rats was achieved
El insecto inyecta formas flageladas llamadas promastigotos, los cuales penetran en macrófagos y células del sistema retículoendotelial donde se convierten en amastigotos
Se estudió la multiplicación de los parásitos en los fibroblastos de las tres especies de roedores infectados tanto por Leishmania infantum como por L. mexicana (cepa OCR) y las diferencias entre las tres fueron estadísticamente significativas (p< 0.05)
Summary
Infection and multiplication of Leishmania infantum and L. mexicana inside of skin fibroblasts from hamsters, mice and rats was achieved. En el presente estudio se desarrolla un modelo experimental in vitro de cultivo de fibroblastos de piel de tres especies de roedores, en contacto con promastigotos de Leishmania mexicana y L. infantum, con el fin de determinar si existen diferencias en cuanto a la susceptibilidad de infección por especies biológicamente disímiles del parásito y si aquellas se relacionan con la constitución genética de los individuos.
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