Abstract
Abstract This manuscript demonstrates an inexpensive method of fabricating 3D dielectrophoretic microfluidic devices using a milling machine equipped with a sub-millimeter end mill. Features were milled into a copperclad substrate, otherwise typically used for printed circuit boards. Milled electrodes themselves serve as walls of the microfluidic channel therefore delivering a stronger electric field throughout the depth of the microchannel compared to traditional, coplanar electrode designs. Dielectrophoretic particle trapping and concentration were demonstrated with 8 μm polystyrene beads at voltages no greater than 10 V. The method of fabrication will be discussed as well as advantages and challenges associated with this technique.
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