Abstract
A simple and efficient procedure for the industrial preparation of akebia saponin D, one of the bioactive compounds commonly found in the well-known Chinese Medicinal herb DipsaciRadix, was developed. First, HPD-722 was selected from among 10 kinds of macroporous absorption resins. Following this step, the purity of akebia saponin D was increased about 10 times from 6.27% to 59.41%. In order to achieve a higher purity, ADS-7 was chosen from among five kinds of macroporous absorption resins, and the purity of akebia saponin D was increased from 59.41% to 95.05%. The result indicated HPD-722 and ADS-7 were the most suitable resins to purify akebia saponin D from DipsaciRadix. Under these conditions, large-scale preparation of akebia saponin D was carried out successfully. The preparation method is simple, efficient, and has been demonstrated to be effective for large scale preparations of akebia saponin D from DipsaciRadix.
Highlights
IntroductionDipsaci Radix, Chinese name Xu Duan, the dried root of Dipsacus asper Wall (Dipsacaceae) widespread in Chinese medicine store in Sichuan Province (China), was first recorded in the ancient pharmaceutical book “Shen Nong Ben Tso Ching”
Dipsaci Radix, Chinese name Xu Duan, the dried root of Dipsacus asper Wall (Dipsacaceae) widespread in Chinese medicine store in Sichuan Province (China), was first recorded in the ancient pharmaceutical book “Shen Nong Ben Tso Ching”and has been used since antiquity to treat bone diseases
Dynamic adsorption tests were conducted on the selected resins HPD-722 and ADS-7, the adsorption kinetic curves were obtained, adsorption equilibration time of ASD on each resin were obtained according to the adsorption kinetic curves
Summary
Dipsaci Radix, Chinese name Xu Duan, the dried root of Dipsacus asper Wall (Dipsacaceae) widespread in China, was first recorded in the ancient pharmaceutical book “Shen Nong Ben Tso Ching”. Molecules 2012, 17 that Dipsaci Radix possesses various bio-active effects, including osteoprotective [6,7], inhibition of Alzheimer’s disease [8], anticomplementary [9], antinociceptive [10] and cytotoxic [11] activities. Akebia saponin D (3-O- -L-arabinopyranosyl hederagenin-28-β-D-glucopyranoside-(1→6)-β-Dglucopyranoside, called asperosaponin VI, ASD, Figure 1), the principal bioactive component in Dipsaci Radix, has been reported to have neuroprotective [12], osteoprotective [13], cardioprotective [14], and apoptosis-inducing effects [15]. The conventional methods to separate ASD from medicinal plants are column chromatography with silica gel [14,15,16,17] and preparative reversed phase HPLC [3] These methods are not suitable for large-scale preparation because of the large amount of solvent wastage, poisonous residual solvents (chloroform and methanol, etc.), high cost, etc. When the separation process was scaled up to the industrial-scale, the purity of ASD was greater than 90%
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