Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon 2,4-D dan BAP dengan Metode in vitro

Abstract

Calamansi fruits ( Citrus microcarpa ) or musk lime is one horticulture crops has been known as beverage and food flavorings. This research aimed to determine the effect of 2,4-D and BAP on callus induction of explants calamansi fruits ( Citrus microcarpa ). Parameters observed is percentage of explants, time appeared callus, callus width, and texture of the callus. Research results and a growing percentage of the width callus explants were analyzed by ANOVA and a further test DMRT at 5% level. The next to appear callus timing parameters and texture of callus analyzed in description. The result shows that a combination of hormones D 4 B 1 and D 4 B 2 produces a callus emerged fastest of 3.3 days after culture. The highest percentage of callus formation that is 100% contained in the treatment of D 2 B 0 , D 2 B 0.5 -D 4 B 0 . The mean width of callus was highest in treatment D 4 B 2 with a width of 0.97 cm. Embryogenic callus resulting by treatment of D 2 B 1 , D 2 B 2 , and D 3 B 0 . However, the best a combination of hormones is treatment D 2 B 2 (2 mg/l, 2,4-D, dan 2 mg/l BAP) producing embryogenic callus edvanced to suspension cultures.