Induction of wheat germ agglutinin synthesis by abscisic and gibberellic acids in roots of wheat seedlings

Abstract

The dynamics of ABA and wheat germ agglutinin (WGA) content and WGA-geneexpression have been studied in roots of 4-d-old wheat seedlings treated with 4 ABA and 0.3 GA. Both hormones caused rapidand significant effects on WGA accumulation, though GA might control thisprocess independently of ABA, since the content of endogenous ABA did not changeafter GA-treatment. The results of dot-blot analysis of transcripts of RNA usingthe 32P labelled WGA-gene as a probe showed that a two-fold increasein WGA content resulted from ABA- and GA-induced activation of WGA-geneexpression.