Induction of transfer-cell formation by iron deficiency in the root epidermis of Helianthus annuus L.

Abstract

Helianthus annuus L. responds to iron deficiency by forming a thickened cortex and abundant root hairs in a zone near the root apex that corresponds to the primary developmental stage. Cytological investigations revealed that within 24 to 48 h of iron deficiency most of the peripheral cells differentiate into transfer cells. The wall labyrinth is always situated on the peripheral walls that face the external medium. The cytoplasm of these cells is characterized by numerous mitochondria, extensive rough endoplasmic reticulum, and large leucoplasts containing protein bodies. These observations are discussed in relation to the fact that Helianthus, as an "iron efficient" plant, responds physiologically to iron deficiency by extrusion of H(+), production of reducing substances, and a steep increase in the uptake efficiency of Fe.