Induction of tracheal stenosis in a rabbit model—endoscopic versus open technique

Abstract

To evaluate and compare open versus endoscopic methods of inducing tracheal stenosis in the New Zealand white rabbit model. Prospective randomized control, pilot study. Fifteen New Zealand white rabbits were used in the study. Induction of tracheal stenosis was performed through an open incision in 6 rabbits, an endoscopic approach was utilized in 8 rabbits, and 1 rabbit served as the control. The open approach was performed by brushing the tracheal mucosa 10 times through an anterior tracheotomy with a nylon brush. The endoscopic method was achieved using a rigid bronchoscope and at a measured distance below the cricoid cartilage, passing the same nylon brush four times in each of four quadrants. Repeat endoscopies for evaluation of stenosis progression were performed at 2 and 3 weeks on all rabbits. In the open group, we observed no stenosis with one mortality as a result of gastroenteritis due to antibiotic use. In the endoscopic group, all animals achieved some degree of stenosis by 14 days, with a mean airway narrowing of 43%. The two deaths in the endoscopic group occurred in the immediate postoperative period secondary to laryngeal edema. The endoscopic procedure was 5 minutes in duration. The endoscopic technique is an effective method of achieving circumferential tracheal stenosis in the rabbit model. Advantages include simple instrumentation, an endoscopic approach, potential for decreased costs, and more reliable results.