Induction of the IL-7 receptor α chain in mouse peripheral B cells by glucocorticoids

Abstract

Expression of the IL-7Rα chain (IL-7Rα) is strictly regulated during development and maturation of lymphocytes. While T cells express the IL-7Rα in the periphery, B cells do not. Glucocorticoids (GCs) have pleiotypic effects on development and function of lymphocytes. Although GCs induce the transcription of IL-7Rα gene in T cells, their effect on B cells is largely unknown. Here, we show that GCs induce the transcription and expression of IL-7Rα in mouse peripheral B cells. This effect does not require de novo protein synthesis, because a protein synthesis inhibitor, cycloheximide, does not block the transcription. IL-7R signal pathway is intact in peripheral B cells because Stat5, one of the signal molecules of the IL-7Rα, is phosphorylated by IL-7 stimulation. We also observed that IL-7 simulation induces the transcription of Cis-1, one of the target genes of Stat5. Furthermore, GC-induced IL-7Rα can transmit survival signal in B cells. Therefore, this study demonstrates that GCs induce the transcription and expression of functional IL-7Rα in peripheral B cells, and implies a potential role of the IL-7R in survival of B cells.