Abstract

The effect of chemicals on the induction of systemic resistance in blackgram (Vigna mungo) against urdbean leaf crinkle virus (ULCV) was studied. Pre-inoculation spraying of blackgram plants with resistance-inducing chemicals, namely salicylic acid and benzothiadiazole at 100 ppm concentration was found to be effective in reducing ULCV infection and increasing the incubation period of the virus under controlled conditions. The treatment of blackgram plants with salicylic acid and benzothiadiazole significantly increased the phenylalanine ammonialyase (PAL), peroxidase (PO) and polyphenol oxidase (PPO) activities. New isoforms of peroxidase and polyphenol oxidase were also induced by these treatments. A two-fold increase in accumulation of total phenol was observed in plants treated with salicylic acid and benzothiadiazole.

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