Abstract
Infection with Brucella abortus causes contagious zoonosis, brucellosis, and leads to abortion in animals and chronic illness in humans. Chitosan nanoparticles (CNs), biocompatible and nontoxic polymers, acts as a mucosal adjuvant. In our previous study, B. abortus malate dehydrogenase (Mdh) was loaded in CNs, and it induced high production of pro-inflammatory cytokines in THP-1 cells and systemic IgA in BALB/C mice. In this study, the time-series gene expression analysis of nasal-associated lymphoid tissue (NALT) was performed to identify the mechanism by which Mdh affect the target site of nasal immunization. We showed that intranasal immunization of CNs-Mdh reduced cell viability of epithelial cells and muscle cells at first 1 h, then induced cellular movement of immune cells such as granulocytes, neutrophils and lymphocytes at 6h, and activated IL-6 signaling pathway at 12h within NALT. These activation of immune cells also promoted signaling pathway for high-mobility group box 1 protein (HMGB1), followed by the maturation of DCs required for mucosal immunity. The CNs also triggered the response to other organism and inflammatory response, showing it is immune-enhancing adjuvant. The ELISA showed that significant production of specific IgA was detected in the fecal excretions and genital secretions from the CNs-Mdh-immunized group after 2 weeks-post immunization. Collectively, these results suggest that B. abortus Mdh-loaded CNs triggers activation of HMGB1, IL-6 and DCs maturation signaling within NALT and induce production of systemic IgG and IgA.
Highlights
Brucellosis is a highly contagious zoonotic disease caused by the genus Brucella, which is a group of gram-negative and facultative intracellular bacteria [1]
To produce B. abortus malate dehydrogenase (Mdh), fusion proteins were constructed with a pCold vector containing a chaperone trigger factor (TF) commonly used to produce soluble and functional proteins
The loading efficiency (LE) was 51.45 ± 3.48% and 78.22 ± 0.75%, which means that 1.54 mg of TF and 2.34 mg of Mdh was loaded per 20 mg of Chitosan nanoparticles (CNs)
Summary
Brucellosis is a highly contagious zoonotic disease caused by the genus Brucella, which is a group of gram-negative and facultative intracellular bacteria [1]. Live attenuated strains of Brucella abortus, such as Rev. 1, S19 and RB51, have been widely used for bovine. Systemic immunity through NALT in the intranasal administration of B. abortus Mdh-loaded nanoparticles at Gene Expression Omnibus (GEO) Ncbi.nlm.nih.gov/geo/) under accession number GSE125765 (https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE125765) Systemic immunity through NALT in the intranasal administration of B. abortus Mdh-loaded nanoparticles at Gene Expression Omnibus (GEO) (https://www. ncbi.nlm.nih.gov/geo/) under accession number GSE125765 (https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE125765)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
