Induction of SOS functions by nitrogen dioxide in Escherichia coli with different DNA-repair capacities

Abstract

The effect of gaseous nitrogen dioxide (NO 2) on cytotoxicity, induction of synthesis of UmuC and RecA proteins, and mutagenesis was studied in Escherichia coli strains with different capacities of DNA repair. Gaseous NO 2 (90, 180 μl/l) killed Escherichia coli. The recA mutant was most sensitive, the lexA mutant moderately sensitive, and the uvrA mutant and the wild-type the least sensitive. When 90 μl/l NO 2 gas was bubbled into bacterial suspensions for 30 min at a flow rate of 100 ml/min, the induction of umuC gene expression increased in the wild-type strain. NO 2 also induced the recA gene expression in the wild-type strain. The synthesis of neither RecA nor UmuC proteins was induced in the recA and lexA mutants. We further investigated the NO 2 mutagenesis in the cells treated with bubbling of NO 2 gas. NO 2 caused mutation to Trp + of WP2.