Induction of Reparative Dentin Following Pulpotomy with Mineral Tri Oxide Aggregate (MTA) One Week Follow up visit: A Case Report

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This study examines whether pulpotomy with mineral trioxide aggreagte could induce reparative dentin formation without eliciting adverse side effects in pulpotomy of premolar teeth, in vivo. Non-carious lower left first premolar tooth was used for pulpotomy with Mineral Trioxide Aggregate (MTA), and the induction of reparative dentin was assessed after one week of extraction. Following mouth preparation of patient, a round shape cavity (diameter: 2 mm) were prepared on the occlusal surface to expose the pulp by using a flat end fissure bur with a high-speed hand piece under sufficient cooling arrangement. Following control of bleeding with sterile cotton pellets, the exposed pulp tissue was covered with Mineral Tri Oxide Aggregate (MTA) according to manufacturer's recommendations. At 1 week, tooth was extracted under local anesthesia and fixed in cold 4% neutral buffered formaldehyde for 24 hours. The specimen was dematerialized and embedded in paraffin. After longitudinal serial sectioning (6µm), every section was stained with haematotoxylin and eosin. Series of sections containing pulp tissue was observed by a blinded observer in a light microscope equipped with a digital camera and computer for histometry. The amount of new hard tissue formed was assessed from experimental tooth. The area covered by newly formed hard tissue in these sections was measured by using digital, histometry equipment. It was found that the thickness of reparative dentin formation is 1.2 ± 0.2µm and completely covers the exposed pulp tissue. There is no pulpal inflammation at 7 weeks.