Induction of Reparative Dentin Following Pulpotomy with Calcium Hydroxide at Four Week Follow up visit: A Case Report

Abstract

This in-vivo study used calcium hydroxide pulpotomy of a premolar tooth to investigate the potential for reparative dentin development without causing unfavorable side effects. For cosmetic reasons, a pulpotomy using calcium hydroxide was used to treat a patient's right first premolar tooth. In order to expose the pulp, a round cavity with a diameter of 2 mm was initially created on the occlusal surface using a flat end fissure bur and a high-speed hand piece under adequate cooling conditions. After using sterile cotton pellets to staunch the bleeding, Dycal was applied to the exposed pulp tissue in accordance with the manufacturer's instructions. The tooth was pulled at 4 weeks under local anesthetic, and it was preserved for 24 hours in 4% neutral buffered formaldehyde. After being demineralized, the specimen was imbedded in paraffin. A blind observer used a light microscope fitted with a digital camera and a computer for histometry to examine a series of sections containing pulp tissue that had been longitudinally serially sectioned at a thickness of 6 µm. lastly, the experimental tooth's amount of newly generated hard tissue was measured. The findings indicated that the exposed pulp tissue is only partially covered by the 0.9 ± 0.2 µm thick layer of reparative dentin development. In conclusion, although it does not entirely cover the exposed pulp, calcium hydroxide can promote reparative dentin production and pulpal lesion healing in exposed teeth.