Abstract
ObjectiveTo establish rat models with myocardial ischemia and anoxia and to detect the expression of succinate receptor (GPR91) in rat myocardium.MethodsTotal 48 Sprague‐Dawley (SD) rats used in the experiment. For the induction of myocardial ischemia, eighteen rats were randomly divided into three groups of sham operation (control), coronary artery ligation 12h and 24h, respectively. The rats were anaesthetized with sodium pentobarbital and ligated by the left anterior descending artery for 12h and 24h. The control rats were operated as the same except ligation. The electrocardiogram (ECG), the area of myocardial infarction and the serum contents of lactate dehydrogenase (LDH), creatine kinase (CK) and cardiac troponin 1 (cTn‐1) in rats were recorded and detected at the setting time. For the induction of myocardial anoxia, thirty rats were divided into five groups of living in normal atmosphere (control), living in low atmosphere for 1d, 3d, 5d, and 7d. At the setting days, the serum contents of lactate LDH, CK and cTn‐1 in rats were detected. Real‐time fluorescent quantitative PCR and Western Blot were used for GPR91 mRNA and protein expression in rat myocardium.ResultsIn the rats ligated by the left anterior descending artery for 12h and 24h, continuous ST segment elevations were recorded in ECG; the ratio of infarct size were 23.18±3.46 and 34.85±2.22 (P<0.05, compared with the control); the serum levels of LDH, CK and cTn‐1 were increased in some extent. In the rats with myocardial anoxia for 1d, 3 d, 5d, and 7d, elevation of LDH, CK and cTn‐1 contents in serum were also detected. Compared with that of the control rats, the expression of GPR91 mRNA and protein in myocardium of myocardial ischemia or anoxia rats were both up‐regulated.ConclusionThe left anterior descending artery ligation or living in low atmosphere could induce myocardial ischemia or anoxia in rats. The expression of GPR91 mRNA and protein in myocardium of rats with myocardial ischemia or anoxia was increased. It implied that GPR91 could serve as a target for prevention and treatment of ischemic or anoxia myocardial injury.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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