Abstract
Nine polyploids (including two mixaploids) were obtained in Trifolium riograndense with three methods of colchicine application to seedlings: immersion, dropping and cotton. The best method showed to be immersion for 6 h in 0.30% colchicine. Leaves with more than 3 folioles were frequently observed in some (but not all) polyploids. The number of pollen grain pores increased from 3 to 4 in tetraploids. Stomatal cell size was not a reliable criterium to distinguish ploidy levels. Two percent of aneuploidy occurred in the C1 generation. Meiotic behavior was characterized by high bivalent and low quadrivalent numbers with suggestions of genetic control of chromosome pairing. Meiotic index ranged from 70% to 98% and pollen viability from 66 to 90%. An apparent tendency to decrease the number of quadrivalents and to increase mean meiotic index and pollen viability from C0 to C1 was detected. The use of these autopolyploids in basic and applied research as well as in genetic breeding is promising.
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