Abstract
Summary Induction of peroxidase isozymes during shoot enhancement on cotyledon explants of loblolly pine by abscisic acid (ABA) was studied using native polyacrylamide gel electrophoresis (PAGE). Unique peroxidase bands were detected when cotyledon explants were cultured in the presence of exogenous ABA. The number of peroxidase bands increased with the length of exposure to ABA. Explants exposed to fluridone (FLU), an inhibitor of ABA biosynthesis, produced fewer bands showing peroxidase isozyme banding patterns that did not vary regardless of the length of treatment. Four weeks of FLU treatment produced the same peroxidase isozyme band pattern as the ABA-treated explants if exogenous ABA was present in the media for the first two weeks of treatment, but not after.
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