Abstract
We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O 2 or H 2O 2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O 2 induced 8OHdG in P. melaninogenica but not in B. fragilis, which shows catalase activity, or in S. typhimurium. In P. melaninogenica, with catalase, O 2 induced less 8OHdG; superoxide dismutase had no effect; with glucose and glucose oxidase, O 2 induced more 8OHdG. H 2O 2 also markedly increased 8OHdG. O 2 was suggested to induce 8OHdG through H 2O 2. O 2 or H 2O 2 decreased survival only in P. melaninogenica. Highly sensitive to oxidative stress, P. melaninogenica could prove useful for investigating oxidative DNA damage.
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