Abstract
This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs.
Highlights
IntroductionMineral trioxide aggregate (MTA) was used as the standard root repair material [1]
For several years, mineral trioxide aggregate (MTA) was used as the standard root repair material [1]
The viability of cultured mesenchymal stem cells (MSCs) varied within the experimental periods according to the effect of
Summary
Mineral trioxide aggregate (MTA) was used as the standard root repair material [1]. MTA is a calcium-silicate-based cement, and it is the material of choice to improve the communication between the dental pulp and periodontal tissues, with clinical as well as histological success [1,2]. The fresh mix of MTA was reported to be biocompatible due to its calcium hydroxide byproduct [3,4], its aged material, cured for 28 days, exhibited reduced cell growth [4]. Many studies reported certain cytotoxicity of MTA on macrophages and fibroblasts [5], and reduced cell growth 28 days after hydration [3]. Several manufacturers developed a new root repair material made from bioceramics
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
