Induction of mutation with colchicine in Olimpus potato by in vitro culture

Abstract

Plant breeding of Olimpus potato is carried out to develop potato varieties which resistant to abiotic stress (high temperature) and to develop potato varieties in the lowland of the tropic. The uses of colchicine is expected to induce genetic diversity as a source for plant breeding. This research aimed to determine the effect of colchicine on the Olimpus potato in vitro and to generates genetic diversity to be developed into new varieties as material lines for selection. This study was conducted using a RBD with two factors, are concentration of colchicine with 5 level are 0%, 0.02%, 0.04%, 0.06%, 0.08% and immersion time duration of 12 h, 24 h and 48 h. The results showed colchicine treatment signifficantlyaffect on vegetative growth variables. Colchicine concentration of treatment with 0.04% and 24 hours immersion showed better growth of shoot than the control at variable leaf number and concentration 0.04% with 48 hours immersion at variable shoot height. Treatment with 0.08% colchicine and 48 hours immersion resulted highest number of internode than the control. The highest number of root present in concentration of colchicine with 0.02% and 12 hours immersion. Colchicine treatment resulted on variations of Olimpus potato shoot differently from control.