Abstract
Induction of metallothionein (MT) in liver of the subtropical fish, bluestriped grunt ( Haemulon sciurus), has been studied after cadmium injection. Hepatic MT was assayed by a heterologous radio immunoassay (RIA) for bluestriped grunt, using antiserum raised against MT from perch ( Perca fluviatilis). Two laboratory experiments were performed: a dose-response experiment and a time-course experiment. Hepatic MT was significantly induced in a dose-dependent manner in response to intraperitoneal injection of CdCl 2. Maximum MT levels were observed in the 2200 μg Cd/kg group, where MT values were three times higher than in the control group. In the time-course experiment, elevated MT levels were found 2 days after injection and the maximum was reached after 6 days. MT values in fish 6 days after treatment were increased five times compared to the control group.
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