Abstract
Summary Mating-type change in the life cycle of Paramecium caudatum is based on intrinsic tem poral programming related to clonal aging. Approximately 80–120 fissions after conjugation, some of the E-type cells change their mating-type to O-type, resulting in an intraclonal mating reaction and in selfing-pair formation. We have found that a natural stock, C103, is a non-selfer mutant with the homozygote of a recessive allele. Microinjection of genomic DNA isolated from selfer strains induced a mating-type change in the non-selfer mutant, C103. These results were consistent with those of previous studies showing that a dominant modifier gene, Su(Mt), suppresses the stable expression of the Mt gene. The present report represents the first demonstration that microinjection of genomic DNA restores the genetic defect related to stable expression of mating type in Paramecium.
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