Abstract
Ligninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strains Daedaleopsis confragosa, Fomes fomentarius, Trametes gibbosa, Trametes suaveolens and Trametes versicolor to produce these enzymes has been studied. Three different copper(II) complexes have been prepared ((Him)[Cu(im)4(H2O)2](btc)·3H2O, where im = imidazole, H3btc = 1,3,5-benzenetricarboxylic acid, [Cu3(pmdien)3(btc)](ClO4)3·6H2O) and [Cu3(mdpta)3(btc)](ClO4)3·4H2O, where pmdien = N,N,N′,N′′,N′′-pentamethyl-diethylenetriamine and mdpta = N,N-bis-(3-aminopropyl)methyl- amine), and their potential application for laccase and peroxidases induction have been tested. The enzyme-inducing activities of the complexes were compared with that of copper sulfate, and it has been found that all of the complexes are suitable for the induction of laccase and peroxidase activities in white-rot fungi; however, the newly-synthesized complex M1 showed the greatest potential for the induction. With respect to the different copper inducers, this parameter seems to be important for enzyme activity, which depends also on the fungal strains.
Highlights
The species of Basidiomycetes are considered to be a very interesting group of fungi, including different ecological groups, such as white-rot, brown-rot and leaf-litter fungi [1]
Daedaleopsis confragosa and Trametes suaveolens exhibited the highest activity of peroxidase enzymes reaching values greater than 14 U/L for lignin peroxidase peroxidase (LiP)
MnP showed very low activity for all fungal strains (Figure 3C), which is in accordance with the outcome of manganese peroxidase secretion by Fomes and Trametes strains [14]
Summary
The species of Basidiomycetes are considered to be a very interesting group of fungi, including different ecological groups, such as white-rot, brown-rot and leaf-litter fungi [1]. The white-rot fungi are able to efficiently decompose lignin due to the production of ligninolytic enzymes, only They produce many other types of enzymes, such as lignin and cellulose degrading enzymes (β-glucosidase, cellobiohydrolase and β-xylosidase), which can interact with the substrate [2]. A higher enzyme activity guarantees a higher and faster transformation of the target substrate and improves the applicability and effectiveness of the enzyme-catalyzed processes [16] From this point of view, the enzymatic induction of white-rot fungi is very important since their metabolic activity and growth are dependent on the environmental conditions. Pentamethyl-diethylenetriamine and mdpta = N,N-bis-(3-aminopropyl)methylamine, and to test (ClO4 )3 ·6H2 O (M2) and [Cu3 (mdpta) (btc)](ClO4 )3 ·4H2 O (M3), where pmdien = N,N,N 0 ,N 00 ,N 00 their ability to increase the enzyme activity of white-rot fungi as inducers.
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