Induction of kopyor coconut embryogenic callus using 2.4-D and TDZ

Abstract

This research was conducted to obtain an effective method to produce true to type coconut kopyor seeds and determine the effect of 2,4-D with or without TDZ in inducing callus to produce somatic embryos. This research was conducted in the Lampung State Polytechnic laboratory with concentrations of 2,4-D 0, 2.5, 5, 7.5, and 10 mg.L-1 with or without the addition of 0.5 mg.L-1. Further analysis of the data used the 5% LSD test. The parameters observed in this study were: percentage of callus explants, callus diameter, callus weight. The results showed that increasing the concentration of 2,4-D and 7.5 mg. L-1 could increase the percentage of embryogenic callus, callus diameter, and callus weight and the addition of 0.5 TDZ concentration to the 2,4-D medium resulted in a decrease in parameters. The best 2,4-D concentration to induce callus embryogenic in kopyor embryos is 7.5 mg. L-1. The use of 2,4-D for embryogenic callus formation can accelerate callus growth and obtain kopyor coconut seeds true to type.