Abstract
Treatment of BALB/c mice with purified pig anti-(BALB/c anti-nuclease) anti-idiotypic antibodies has been found to induce the appearance of idiotype-bearing immunoglobulins (Id') in the serum of these mice in the absence of detectable antigen binding activity. This phenomenon appeared to require T cells in the hosts because no Id' was detected in the serum of nude mice similarly treated. Furthermore, the spleens of BALB/c mice treated with anti-idiotype were found to contain helper T cells capable of providing help in an in vitro plaque-forming cell response to trinitrophenyl-nuclease equivalent to that provided by helper T cells from the spleens of nuclease-primed animals. Helper T cells from both anti-idiotype-treated and nuclease-treated animals were found to be antigen-specific and to be similarly susceptible to elimination by treatment with anti-idiotype plus complement. Therefore, treatment with both antigen and anti-idiotype appeared to prime similar populations of antigen-specific helper T cells, while having different effects on the induction of antibody. These findings are consistent with the network theory of receptor interactions in the immune response, and may provide a means for studying individual cell populations involved in such interactions.
Highlights
MethodsBALB/cAnN and BALB/cAnN nude mice were obtained from the Animal Production Unit, National Cancer Institute
B A L B / c nude mice, failed to produce Id' after anti-Id administered either as an emulsion in complete Freund's adjuvant (CFA) or in solution in saline. These results suggested that direct B cell activation by anti-Id, if it occurs, is insufficient to generate detectable quantities of these molecules and that functional T cells played a role in the generation of such molecules
We can say with assurance only that a subpopulation of T cells exists in animals primed with anti-Id that is antigen-specific and bears idiotypic determinants on its surface
Summary
BALB/cAnN and BALB/cAnN nude mice were obtained from the Animal Production Unit, National Cancer Institute. A/J and A.BY mice were obtained from The Jackson Laboratory (Bar Harbor, Maine). Miniature swine were bred and housed at the National Institutes of Health Animal Facility, Poolesville, Md. Antigens. Nuclease was purified as previously published [8] or purchased from the Boehringer-Mannheim Biochemical Co. We used nuclease purified in our laboratory for the immunization of animals and the commerical preparation for conjugation to 2,4,6-trinitrobenzene sulfonie acid (TNBS) and in vitro challenge. Nuclease and K L H were conjugated with TNBS as previously described [9], at ratios of 17.4 and 13.0 trinitrophenyl (TNP) residues per 100,000 tool wt, respectively
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