Abstract
Glioblastoma (GBM) is an aggressive brain tumor in humans. The median survival rate of patients is one year after the diagnosis. So, development of an animal model is necessary for the advances in the research treatment of GBM. The aim of this study was to investigate the capability of human glioma cells in inducing glioma tumors in rats with intact immune system. U87 cells were implanted in the frontal lobe of rats without suppressing the immune system. We used magnetic resonance imaging (MRI), Hematoxylin-Eosin (H&E) and Immunohistochemical (IHC) staining to assess characteristics of tumor. At the 10th and 14th days of tumor inoculation, MRI images contained the tumor areas in the brain. All tumor-bearing rats developed tumors. The rats retained the morphology and histological characteristics of human glioma. Animals mimic GBM characteristics, such as mitotic activity, invasion, neovascularization, necrosis and pseudopalisading cells. IHC staining revealed tumor growth and progression in the tumor-bearing rats. This model is a standard system for studying the tumor phenotype, genotype, and for evaluating the efficacy of anti-cancer agents. It is a reliable, simple, inexpensive, and easily reproducible model, which may be a way for pre-clinical studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.