Abstract
Methods Peripheral blood mononuclear cells (PBMC) from baboons primed with MVA and boosted with VLPs (n=3) or vaccinated with VLPs only controls (n=2) were stimulated with HIV-1 Gag peptide pools. T cell cytokine production (multiplex TNF-a, IFN-y and IL-2) and memory phenotype was determined by flow cytometry. Human anti-CD28 and CD95 antibodies were used to delineate effector memory (Tem) and central memory (Tcm) T cells.
Highlights
We previously reported induction of HIV-specific responses in Chacma baboons following immunization with SAAVI MVA-C (MVA) and HIV-1 Pr55 Gag viruslike particles (VLPs) in a prime-boost vaccination strategy
Peripheral blood mononuclear cells (PBMC) from baboons primed with MVA and boosted with VLPs (n=3) or vaccinated with VLPs only controls (n=2) were stimulated with HIV-1 Gag peptide pools
Gag-specific CD4+ cells from the prime-boost animals were significantly skewed towards a Tcm phenotype (>95%) of total cytokine responses compared to the to delineate effector memory (Tem) phenotype (
Summary
We previously reported induction of HIV-specific responses in Chacma baboons following immunization with SAAVI MVA-C (MVA) and HIV-1 Pr55 Gag viruslike particles (VLPs) in a prime-boost vaccination strategy. We characterised the vaccine specific memory T cells by flow cytometry
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