Abstract
Background. Magnetic hyperthermia using superparamagnetic nanoparticle (SPNP) agents is considered a promising biotechnological approach to induce heat shock proteins (HSPs) in a target tissue because it can generate accurately controllable localized heating.Objectives. The main objective of this study is to demonstrate induction of HSPs in cultured retinal ganglion cells (RGCs) by using engineered Mn0.5Zn0.5Fe2O4SPNP agents coated with polyethylene glycol (PEG) 500.Methods. The Mn0.5Zn0.5Fe2O4nanoparticles were synthesized using a high temperature thermal decomposition method. The AC heating characteristics of PEG 500-coated Mn0.5Zn0.5Fe2O4nanoparticles were investigated using an AC solenoid coil-capacitor system.Results. PEG 500-coated SPNPs efficiently penetrated into the cytoplasm of RGCs without causing obvious cytological changes and showed stable and well-saturated self-heating temperature rise characteristics. Immunofluorescent staining images showed that AC magnetic hyperthermia successfully induced HSP72 in RGCs incubated with Mn0.5Zn0.5Fe2O4nanoparticles. In Western blot analysis, a significant increase in immunoreactivity was observed for RGCs incubated with SPNPs in a fixed AC magnetic field (fappl=140 kHz andHappl=140 Oe).Conclusion. Our results demonstrate that the induction of HSP72 with a magnetic nanofluid hyperthermia could potentially be used as a neuroprotective treatment modality by way of enhancing a natural cytoprotective response.
Highlights
Glaucoma is characterized by progressive optic nerve damage with selective loss of retinal ganglion cells (RGCs) [1,2,3]
In order to investigate the AC magnetically induced heating characteristics in RGCs, we developed an AC magnetic field generation system, which consists of AC coils, capacitors, DC power supplies, and wave generators
We employed a localized magnetic hyperthermia system by using the superparamagnetic nanoparticle (SPNP) agents as a new nanobiotechnological approach to effectively control the local induction of HSP72
Summary
Glaucoma is characterized by progressive optic nerve damage with selective loss of retinal ganglion cells (RGCs) [1,2,3]. From this background, the importance of neuroprotection in the treatment of glaucoma has become a worldwide issue. Many studies have focused on discovering neuroprotective agents along with IOP lowering medications in order to prevent retinal ganglion cell (RGC) death or even reverse the process of cell death [4]. The main objective of this study is to demonstrate induction of HSPs in cultured retinal ganglion cells (RGCs) by using engineered Mn0.5Zn0.5Fe2O4 SPNP agents coated with polyethylene glycol (PEG) 500. Immunofluorescent staining images showed that AC magnetic hyperthermia successfully induced HSP72 in RGCs incubated with Mn0.5Zn0.5Fe2O4 nanoparticles. Our results demonstrate that the induction of HSP72 with a magnetic nanofluid hyperthermia could potentially be used as a neuroprotective treatment modality by way of enhancing a natural cytoprotective response
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