Abstract
We have studied the effect of RNA synthesis inhibitors, such as Actinomycin D, α-Amanitin, or DRB, on the heat-inducible puffs of Chironomus thummi. While the appearance of most of the T-puffs is blocked by the inhibitory treatments, the induction of a heat-shock Balbiani Ring (T-BR-III) takes place in the presence of these drugs. No transcriptional activity could be detected in this structure. However, its frequency and approximate size values are similar to those obtained for T-BR-IIIs induced under normal conditions. Accumulation of acidic proteins also takes place in T-BR-IIIs in the presence of these inhibitors. On the other hand, puff III-A3b, a major T-puff of Ch. thummi, seems to be resistent to DRB treatment at both the puffing and the transcriptional levels. These results are discussed in the context of puffing-RNA synthesis relationships.
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