Abstract

Gynogenesis was induced in silver carp, Hypophthalmichthys molitrix, to test female homogamety for sex determination and subsequently, to produce genetic females for androgen sex reversal. Heat shock (34°C) slightly increased the yield of gynogenetic diploids, but mild cold shock (22°C) was less productive than no-shock controls and severe cold shock (5°C) was lethal. All gynogens were females. Treatment with methyltestosterone suspended in liquid dimethylpolysiloxane was ineffective in inverting the phenotypic sex of silver carp but the androgen delivered from a silastic implant induced sex reversal of genetic females in some treatments. Males were produced when treatment was initiated at 79 days of age (110–120 or 125–135 mm, TL), 124 days of age (100–110 mm, TL) and 319 days of age (90–100 mm, TL). The period of gonadal differentiation was affected by growth rate and was correlated with successful reversal.

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