Abstract
Glucoraphasatin (GRH) is a specific aliphatic glucosinolate (GSL) that is only abundant in radish (Raphanus sativus L.). The gene expression regulating GRH biosynthesis in radish is still poorly understood. We employed a total of 59 radish accessions to analyze GSL profiles and showed that GRH was specific and predominant among the aliphatic GSLs in radish roots. We selected five accessions roots with high, moderate and low GSL biosynthesis, respectively, to conduct a comparative transcriptome analysis and the qRT-PCR of the biosynthesis genes for aliphatic GSLs. In this study, among all the accessions tested, roots with the accession RA157-74 had a high GRH content and showed a significant expression of the aliphatic GSL biosynthesis genes. We defined the genes involved in the GRH biosynthesis process and found that they were regulated by a transcription factor (RSG00789) at the MYB29 locus in radish roots. We found 13 aliphatic GSL biosynthesis genes regulated by the RSG00789 gene in the GRH biosynthesis pathway.
Highlights
Glucosinolates (GSLs) are secondary metabolites containing nitrogen and sulfur, mainly found in plants
Major genes involved in GSL biosynthesis have been reported in the Brassicaceae family, including in Arabidopsis thaliana, two Brassica rapa, three Brassica oleracea and Raphanus sativus [2,4,5,6,7,8,9,10,11,12]
MYB28 and MYB29 are transcription factors that regulate the genes involved in aliphatic GSL biosynthesis [6]
Summary
Glucosinolates (GSLs) are secondary metabolites containing nitrogen and sulfur, mainly found in plants. Major genes involved in GSL biosynthesis have been reported in the Brassicaceae family, including in Arabidopsis thaliana, two Brassica rapa GRH is important as a biological and potential health promoting compound, GSL metabolism is mainly studied in broccoli, kale, cabbage and Chinese cabbage. The analysis of RNA sequencing and quantitative RT-PCR (qRT-PCR) showed that major genes involved in GRH biosynthesis were strongly regulated by MYB29, which was not dependent on the GRH content of the radish roots. We investigated the MYB inducible mechanism of GRH biosynthesis in radish roots and identified those accessions that had a high GRH biosynthesis These results may be useful in order to breed valuable new radish cultivars because the GRH content is an important and major trait in radishes
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