Abstract
IN reviews of the control of fertilization in flowering plants, experimental methods of overcoming self-incompatibility have been discussed1,2. For example, induction of auto-tetraploidy by colchicine produced compatibility in a diploid self-incompatible species of Petunia3, but self-pollination of the excised whole pistils cultured in vitro did not, as one of us has shown4. We report here the elimination of self-incompatibility in the diploid (n = 7) Petunia axillaris (Lam.) B.S.P. by fertilization in vitro, a technique devised in this laboratory5 and improved by us.
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