Induction of expression of LAT1, an essential amino acid transporter, in activated T cells (P1182)

Abstract

Abstract Activation of T cell accompanies remarkable enhancement of metabolism. Sufficient and continuous supply of nutrient such as amino acid is thus considerable importance to support immune reaction in T cell. However, the molecular mechanism of efficient incorporation of amino acid into activated T cell has not been determined. We characterized LAT1 as an essential amino acid transporter which is highly expressed in activated T cell. TCR stimulation and co-stimulation of human T cell triggered the dramatic induction of LAT1, although neither TCR stimulation nor co-stimulation alone did not affect the LAT1 expression. The promotion of LAT1 expression in activated T cell was prohibited by inhibitor of NF-kB and AP-1. These results indicate that LAT1 expression is induced by full activation of T cell and LAT1 could work as a potential transporter for essential amino acid in activated T cell. Our result that NF-kB and AP-1 are essential for LAT1 expression suggests that nutrient supply system and immune reaction system are included in one program and those systems are activated by the same molecular machinery in T cell.