INDUCTION OF ESTRUS IN FEMALE DOGS BY GONADOTROPIN RELEASING HORMONE IN THE ANESTHETIC PERIOD

Abstract

The article presents the results of an experiment conducted on two groups of dogs with the aim of inducing estrus in the anestral period by using gonadotropin-releasing hormone. As the drug GnRH we used Surfagon. Experimental animals were represented by two groups of 6 animals each. To the dogs of the first group, the GnRH Surfagon preparation was administered at a dose of 0.6 μg / kg body weight twice a day with a 48 hour interval, the second group was administered the drug at a dose of 0.6 μg / kg twice a day for 3 days continu-ously. Effect of the preparations was assessed by the complex of clinical signs, the cytological picture of smears-prints obtained from the surface of the vaginal mucosa. A stable response to the administration of the GnRH surfactant was found in females of the first group, which on the fourteenth day from the start of the ex-periment showed typical signs of late proestrus and the onset of estrus. In the second group, signs of estrus were observed in 4 dogs out of 6. On the eighteenth day from the start of GnRH, all animals from the first group and four from the second showed clear clinical signs of readiness for breeding. In smears-prints ob-tained from the mucous membrane of vagina in females of the first group, basal cells were absent, the number of intermediate cells did not exceed 3%, and the content of the super-formal reached 97% of the cellular com-position. nmol / l in animals of the first group and up to 23.8 ± 5.1 nmol / l in the second. The results suggest that the GnRH surfactant at a dose of 0.6 μg / kg body weight, applied twice a day with a 48-hour interval three times is able to cause estrus in dogs in the anestral period.