Abstract
AbstractDuring the induction of DNA synthesis in Jerusalem artichoke (Helianthus tuberosus L.) tuber by 2,4‐D, the 2‐14C‐2, 4‐D from the agar medium rapidly incorporated into the ethanol soluble and insoluble fractions. Although the 2,4‐D level in the ethanol soluble fraction decreased on transplantation of the tissue from the 2‐14C‐2,4‐D medium to medium without the auxin, its level in the buffer‐soluble and ‐insoluble macromolecular fractions increased. The purified, buffer‐insoluble macromolecules were chromatin. The 2,4‐D binding to chromatin particularly increased during DNA synthesis. The histone contents of chromatin decreased as DNA synthesis progressed. The polyacrylamide gel electrophoretic patterns of the histones showed a decrease in the moderately lysine‐rich histone fraction as compared to other fractions. Thus, the decrease in the histone level caused by 2,4‐D and the presence of the 2,4‐D moderately lysine‐rich histone complex may be closely related to the induction of DNA synthesis by 2,4‐D in cells.
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