Induction of different types of callus in Jatropha curcas L. hybrid accession at in vitro condition

Abstract

Micropropagation protocols depend on several factors, particularly on the types, combinations, and levels of plant growth regulators used in the culture media. The aim of this study was to determine the influence of 6-Benzylaminopurine (BAP) and Naphtalen-3-acetic acid (NAA) concentrations on callus induction of Jatropha curcas L. explants. The plant material used was a jatropha hybrid accession developed through intraspecific crosses between Costa Rica and Mexico genetic resources (TREC 49 x TREC 33 accession codes). Callus cultures were initiated from hypocotyl explants isolated from 15-days-old seedlings, on Murashige and Skoog (MS) basal medium supplemented with 0; 2 or 4 mg L-1 BAP and 0; 1 or 2 mg L-1 NAA in different combinations. Better-quality callus formation was obtained in medium supplemented with BAP 2 mg L-1 and NAA 1 mg L-1. This combination of plant growth regulators resulted in higher amount of white colored and friable calli, which are more likely to differentiate in bud shoots and roots, depending upon the plant growth regulations added to the media. Our results suggest that BAP (2 mg L-1) and NAA (1 mg L-1) combined can be used for in vitro callogenesis of J. curcas hybrid accession and that it can be adapted to a large-scale in vitro mass production system or to support biotechnological protocols.